Journal: iScience

Article Title: Lipid-based mRNA chimeric antigen receptor T cells for enhanced in vitro antitumor activity

doi: 10.1016/j.isci.2026.117390

Figure Lengend Snippet: In vitro antitumor efficacy and effector function of mRNA CAR T cells (A) Enlarged schematic of the real-time cytotoxicity assessment (RTCA) workflow. (B) Cytolysis of MDA-MB-468 cells at 24 h across various E:T ratios. (C and D) Cytokine secretion (IL-2, TNF-α, IFN-γ, and CD4/CD8 subset distribution). (E–G) Flow cytometry analysis of exhaustion markers. (H) Schematic illustration of the mechanism for mRNA-CAR delivery into T cells via lipid nanoparticles. (I) Cytolysis of SKOV3 cells at 24 h across various E:T ratios. (J) Supernatant cytokine secretion (IL-2, TNF-α, and IFN-γ) following coculture with SKOV3 cells. (K–N) Flow cytometry analysis of CD4/CD8 T cell subset distribution and exhaustion markers (LAG-3, TIM-3 and PD-1) on T cells over time in SKOV3 cocultures. Data represent mean ± SD from three independent biological donors. Data are presented as mean ± SD, n = 3 (independent biological replicates). The statistical analysis was measured by paired two-tailed t tests. ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p ≤ 0.0001, and ns indicates no significance.

Article Snippet: The SKOV3 cell line was purchased from ATCC, while the DC2.4 and MDA-MB-468 cell lines were obtained from the Cell Bank of Chinese Academy of Sciences.

Techniques: In Vitro, Flow Cytometry, Analysis, Two Tailed Test

Journal: iScience

Article Title: Lipid-based mRNA chimeric antigen receptor T cells for enhanced in vitro antitumor activity

doi: 10.1016/j.isci.2026.117390

Figure Lengend Snippet:

Article Snippet: The SKOV3 cell line was purchased from ATCC, while the DC2.4 and MDA-MB-468 cell lines were obtained from the Cell Bank of Chinese Academy of Sciences.

Techniques: Recombinant, Saline, Modification, Virus, Filtration, Membrane, Cell Isolation, Software, FCAP Assay, Sequencing

Journal: International Journal of Genomics

Article Title: Single‐Cell Multiomics Integration Reveals Cell Trajectories and Regulatory Networks in Breast Cancer Progression

doi: 10.1155/ijog/9324717

Figure Lengend Snippet: Quantitative RT‐PCR validation of key subtype marker genes in breast cancer cell lines. Bar chart of relative mRNA expression levels of six marker genes (ERBB2, ESR1, PGR, KRT5, VIM, and CDH1) normalized to GAPDH in MCF‐7 (luminal, blue bars) and MDA‐MB‐231 (basal/TNBC, red bars) cell lines. Error bars represent standard deviation from three independent biological replicates. Significance brackets indicate: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by two‐tailed Student′s t ‐test. Luminal markers ERBB2, ESR1, PGR, and CDH1 show significantly higher expression in MCF‐7, whereas basal/mesenchymal markers KRT5 and VIM are significantly enriched in MDA‐MB‐231, validating single‐cell sequencing findings.

Article Snippet: MCF‐7 (luminal, ER+/PR+/HER2‐, ATCC HTB‐22) and MDA‐MB‐231 (basal/TNBC, ATCC HTB‐26) cell lines were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 μ g/mL streptomycin at 37°C/5% CO2.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Marker, Expressing, Standard Deviation, Two Tailed Test, Single-Cell Sequencing